yrkL [2018-02-08T01:54:15.000Z]

similar to NAD(P)H oxidoreductase

locus: BSU26470

pI: 5.36

mw: 19.95 kDa

protein length: 174 aa

gene length: 522 bp

function: unknown

product: unknown

essential: no

synonyms

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.1|Electron transport and ATP synthesis] → [category|SW 2.1.4|Electron transport/ other/ based on similarity]

Gene

Coordinates: 2,708,175 → 2,708,699

The protein

Protein family

• NAD(P)H dehydrogenase (quinone) family (according to Swiss-Prot)

Paralogous protein(s)

• [protein|2782BD764716CF5FAD0988284310CF75508F2286|YdeQ], [protein|E8048E181756BCE307CF28CF31664421404748A4|YwrO]

Structure

• [PDB|5LVA] (from Streptomyces violaceus, 55% identity) [pubmed|27714222]

Expression and Regulation

Operons

• genes: [gene|1BEBD09273240B6B50DABCB160FCCBC205BB2AAE|yrkK]-[gene|2EA9792C1C7CD89E43742F57FFB7300725B6AEF4|yrkL]
  description: [pubmed|22383849]

• genes: [gene|search|yrkK ]-[gene|2EA9792C1C7CD89E43742F57FFB7300725B6AEF4|yrkL]
  description: [pubmed|22383849]

Biological materials

Mutant

• MGNA-C505 (yrkL::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2503 NBRP B. subtilis, Japan]
• BKE26470 (Δ[gene|2EA9792C1C7CD89E43742F57FFB7300725B6AEF4|yrkL]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE26470 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTAGACTGATTATCCTTT, downstream forward: _UP4_TAATCCGGTATTCGCGAGGG
• BKK26470 (Δ[gene|2EA9792C1C7CD89E43742F57FFB7300725B6AEF4|yrkL]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK26470 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTAGACTGATTATCCTTT, downstream forward: _UP4_TAATCCGGTATTCGCGAGGG

References

Research papers

• 27714222